Detection of Apoptosis Based on the Interaction between Annexin V and Phosphatidylserine

摘要

The detection of apoptosis based on the interaction between annexin V and phosphatidylserine is presented in this paper. The in vitro experimental results demonstrate that annexin V in polyethylenimine film can maintain its high affinity with phosphatidylserine translocated from the inner to the outer plasma membrane of the apoptotic cells, which may consequently inhibit the redox reaction of [Ru(NH_(3))_(5)Cl]~(2+/+) at the annexin V and polyethylenimine comodified pyrolytic graphite electrode. Therefore, if Ca~(2+), the required ion for the function of annexin V, is added into the test solution, the redox wave of [Ru(NH_(3))_(5)Cl]~(2+/+) can be clearly changed to indicate apoptosis and a simple and convenient method to detect apoptosis is developed.