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Impedance Biosensor for Peanut Protein Ara h 1

机译:花生蛋白Ara h 1的阻抗生物传感器

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A reagentless electrochemical impedance biosensor for detection of peanut protein Ara h 1, one of the allergenic proteins found in peanuts, has been demonstrated using an Au substrate onto which an antibody film has been immobilized. Following initial stabilization of the self-assembled monolayer (SAM) through which the antibody is immobilized, the biosensor substrate exhibits stable impedance spectra at different stages of substrate preparation. By fitting the impedance spectra to a Randles equivalent circuit, one can demonstrate that the charge-transfer resistance (R_(ct)) increases and the differential capacitance (C_(d)) decreases with increasing concentration of Ara h 1, although R_(ct) exhibits greater sensitivity. The detection limit of this reagentless biosensor is estimated to be less than 0.3 nM. Assuming a Langmuir adsorption isotherm, the dissociation constant of the peanut protein Ara h 1 and its antibody can be calculated as 0.52 nM from the variation in differential capacitance with Ara h 1 concentration.
机译:已经证明,使用Au底物固定了抗体膜,该试剂可检测花生蛋白Ara h 1(一种在花生中发现的致敏蛋白)的无试剂电化学阻抗生物传感器。在通过固定化抗体的自组装单分子层(SAM)进行初步稳定之后,生物传感器基质在基质制备的不同阶段显示出稳定的阻抗谱。通过将阻抗谱拟合到Randles等效电路,可以证明,随着Ara h 1浓度的增加,电荷转移电阻(R_(ct))增大,而差分电容(C_(d))减小,尽管R_(ct )具有更高的灵敏度。该无试剂生物传感器的检测极限估计小于0.3 nM。假设Langmuir吸附等温线,则花生蛋白Ara h 1及其抗体的解离常数可以根据Ara h 1浓度的差动电容变化算出0.52 nM。

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