STED microscopy is adapted to achieve video-rate imaging of live-cell dynamics. Investigation of the chemical and physical details of living cells has advanced dramatically in recent years. But to date, direct nanoscopic observation of intracellular dynamics has remained beyond reach. Now, Stefan Hell and colleagues at the Max Planck Institute for Biophysical Chemistry and the European Neuroscience Institute (both in Germany) have developed an optical system capable of video-rate (28 frames/s) imaging with <62 nm resolution (Science 2008, DOI 10.1126/science.1154228). Hell says that for many years, high-resolution imaging capability and real-time imaging of physiological processes in a live cell seemed incompatible. In their new method, Hell and colleagues show that optical microscopy can quickly image cells with high resolution. "I feel this will change the way people think about light microscopy," Hell says.
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机译：STED显微镜适用于实现活细胞动力学的视频速率成像。近年来，对活细胞化学和物理细节的研究取得了巨大进展。但是迄今为止，细胞内动力学的直接纳米观察仍然遥不可及。现在，马克斯·普朗克生物物理化学研究所和欧洲神经科学研究所（均为德国）的Stefan Hell及其同事开发了一种光学系统，该系统能够以低于62 nm的分辨率对视频速率（28帧/秒）成像（Science 2008， DOI 10.1126 / science.1154228）。地狱说，多年来，高分辨率成像能力和活细胞中生理过程的实时成像似乎不兼容。 Hell及其同事在他们的新方法中表明，光学显微镜可以快速对高分辨率的细胞成像。赫尔说：“我认为这将改变人们对光学显微镜的看法。”
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