THE PROMISE OF PHAGE DISPLAY: Customized Affinity and Specificity

摘要

Phage display offers readily tailored binding affinity for a wide range of analytes, resulting in a capability that can be increasingly exploited. Before a biosensor can detect a molecule, it must concentrate this molecule within a bioaffinity matrix consisting of a support (often porous) decorated with receptors. These receptors recognize and bind the target molecules of interest. For biosensors, monoclonal antibodies have been the gold-standard receptors in terms of affinity and selectivity for 30 years (1). Researchers favor antibodies for several reasons, including their wide availability and robustness.