Lipid Bilayer Microarray for Parallel Recording of Transmembrane Ion Currents

摘要

This paper describes a multiwell biochip for simultaneous parallel recording of ion current through transmembrane pores reconstituted in planar lipid bilayer arrays.Use of a thin poly(p-xylylene) (parylene) film having micrometer-sized apertures (phi=15-50 mu m,t=20 mu m) led to formation of highly stable bilayer lipid membranes (BLMs) for incorporation of transmembrane pores;thus,a large number of BLMs could be arrayed without any skillful technique.We optically confirmed the simultaneous formation of BLMs in a 5 x 5 matrix,and in our durability test,the BIM lasted more than 15 h.Simultaneous parallel recording of alamethicin and gramicidin transmembrane pores in multiple contiguous recording sites demonstrated the feasibility of high-throughput screening of transmembrane ion currents in artificial lipid bilayers.