A New Look at the Selectivity of RPC Columns

摘要

Bonded-phase columns for reversed-phase chromatography(RPC),also called reversed-phase HPLC,are widely used by chemists and biochemists for analysis and purification.Since their introduction in the early 1970s,these columns have continued to inspire new research aimed at a better understanding of their nature and use(1-5).The behavior of these columns can be described in terms of their kinetic and equilibrium properties.The contribution of column kinetics to chromatographic separation determines the width of resulting peaks in a chromatogram;column kinetics and widths can be described by the column plate number.The dependence of plate number and width on such experimental conditions as column length,particle size,flow rate,and other variables is now well understood(6).