Screening and Identification of Organophosphorus Compounds Related to the Chemical Weapons Convention with 1D and 2D NMR Spectroscopy

摘要

Two-dimensional ~(1)H-~(31)P Fast-HMQC was tested for determination of the presence in low concentrations of organophosphorus compounds related to the Chemical Weapons Convention. This method, based on inverse detection, demonstrated high sensitivity and selectivity. Background signals, such as solvent peaks, are suppressed with good efficiency, and organophosphorus compounds present at a concentration level 1-10 (mu)g/mL can be detected within a few hours. In addition, phosphorus-selective one-dimensional ~(1)H-~(31)P HSQC-TOCSY was applied to produce a complete proton spectrum of selected organophosphorus compound from a sample containing intense background resonances. Application of the methods presented in this paper resulted in considerably improved performance of NMR spectroscopy as a complementary technique for screening as well as identification of chemical warfare agents in environmental samples.