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Sheathless Capillary Electrophoresis/Electrospray lonization Mass Spectrometry Using a Pulled Bare Fused-Silica Capillary as the Electrospray Emitter

机译:使用裸金属熔融石英毛细管作为电喷雾发射器的无鞘毛细管电泳/电喷雾电离质谱

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It has always been assumed that electrical contact at the capillary outlet is a necessary requirement when coupling capillary electrophoresis(CE)with electrospray ionization mass spectrometry(ESI-MS).In this study,we used a pulled bare-capillary tip as the ESI emitter,but neither was it coated with any electrically conductive materials nor was a high external voltage applied on its outlet In this paper,we demonstrate that this straightforward approach may be used to generate multiply charged ions of proteins and peptides through electrospray ionization.Our results indicate that peptides and proteins,including bradykinin,cytochrome c,myoglobin,and tryptic digest products that elute from a pulled bare-capillary tip can be detected directly by ESI-MS using the tapered bare-capillary interface.Thus,we have demonstrated that CE and ESI-MS may be combined successfully without the need to modify the outlet of the capillary tip with an electrically contacting material.
机译:一直认为,将毛细管电泳(CE)与电喷雾电离质谱(ESI-MS)耦合时,必须在毛细管出口处进行电接触。在本研究中,我们使用拉动的裸毛细管尖端作为ESI发射极但是,它既未涂有任何导电材料,也未在其出口上施加高的外部电压。在本文中,我们证明了这种简单的方法可用于通过电喷雾电离产生蛋白质和多肽的多电荷离子。我们的结果表明ESI-MS可使用锥形的裸毛细管界面直接检测从拉动的裸毛细管尖端洗脱的肽和蛋白质,包括缓激肽,细胞色素c,肌红蛋白和胰蛋白酶消化产物。因此,我们证明了CE和ESI-MS可以成功组合,而无需使用电接触材料修改毛细管尖端的出口。

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