Quantum-Dot-Based FRET Detection of Histone Acetyltransferase Activity

摘要

We have demonstrated that QDots can serve as efficacious reagents for the detection of HAT activity in a simple, convenient homogeneous FRET assay. We anticipate that this approach should be sufficiently versatile to provide a general platform to study the activity of other histone-modifying enzymes by simply changing the sequence of the substrate peptide and complementary modification-specific antibody. These assays may be used in drug-discovery applications to identify novel small-molecule regulators of epigenetic enzymes.