Trapping of a Chromophoric Intermediate in the Pdx1-Catalyzed Biosynthesis of Pyridoxal 5'-Phosphate

摘要

Pyridoxal 5'-phosphate(PLP,1)is the biologically active form of vitamin B6,and is essential for performing the chemistry of primary metabolism in all varieties of life.The most common pathway for the biosynthesis of PLP involves only two enzymes,Pdx1(SNZ)and Pdx2(SNO).This biosynthetic activity has recently been reconstituted in vitro and was shown to use ribose-5-phosphate(R5P,2),glutamine 3,and glyceraldehyde-3-phosphate(G3P,4)as substrates(Scheme 1).