Heterocoagulation of Polysaccharide-Coated Platinum Nanoparticles with Ovarian-Cancer Cells

摘要

Platinum nanoparticles coated with adsorption layers of polysaccharides of Chlorella vulgaris (Pt~(PS)) have been synthesized and their heterocoagulation with human ovarian-cancer cells of the A2780 line has been studied. It has been shown that the characteristic time of the aggregation of the cells with Pt~(PS) nano- particles is 20–25 min, which corresponds to fast barrierless coagulation. The cytotoxic effect of Pt~(PS) nano- particles reaches saturation and more than 50% of the cells die within this time period. Cytomorphological investigations have shown that A2780 cells with doubled resistance to an antitumoral antibiotic, doxorubicin, are more sensitive to the toxic action of Pt~(PS) nanoparticles than nonresistant cells are. An electrokinetic con- trol test has been proposed for cancer-cell resistance based on the measurement of variations in the ζ-poten- tial of nanoparticles due to adsorption of water-soluble metabolites of the cells on a particle surface.