P2X_7 deficiency attenuates hypertension and renal injury in deoxycorticosterone acetate-salt hypertension

摘要

Experimental animals. Male WT (C57BL/6J) mice were obtained from SLC Japan (Shizuoka, Japan), and male P2X7 KO mice (26) were obtained from Jackson Laboratory (Bar Harbor, ME). P2X7 KO mice were congenic with the C57BL/6J genetic background. The animals used in the experiments were 10-12 wk old. The mice were housed in a temperature-controlled pathogen-free room with light from 0700 to 1900 (daytime) and had free access to food and water. The experimental protocols were approved by the National Cerebral and Cardiovascular Center Committee for Laboratory Animals, and the care and use of the animals were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals.