Cross-species amplification of microsatellite markers in Bambara groundnut (Vigna subterranean) and their application in diversity study.

摘要

Although Bambara groundnut is an important traditional legume crop of Africa, research on genomics and molecular breeding is very limited due mainly to the lack of efficient DNA markers. In this study, we investigated the use of microsatellite markers from other legumes in this crop. DNA of eight Bambara groundnut accessions was amplified with 147 microsatellite markers: 45 from cowpea; 43 from mungbean; and 59 from azuki bean. 116 markers (78.91%) gave clear PCR products. 88.67, 83.72 and 69.49% of the markers from cowpea, mungbean and azuki bean successfully amplified the DNA of Bambara groundnut, respectively. When eight of these microsatellite markers were used to study diversity of 34 Bambara groundnut accessions, seven showed polymorphism. The number of alleles per locus ranged from 2 to 8 with an average of 3.57, gene diversity ranged from 0.16 to 0.76 with an average of 0.49, and polymorphism information content from 0.16 to 0.73 with an average of 0.42. UPGMA cluster analysis grouped the germplasm into three major groups and one outlier. Principal component analysis revealed an unclear relationship among germplasm.