Identification of a new white-berried grapevine cultivar as a result from bud sport of the Portuguese blue black cultivar 'Alfrocheiro Preto'.

摘要

Many mutations affecting berry colour in Vitis vinifera cultivars have been reported. In the Portuguese National Ampelographic Collection at INIA - Dois Portos, an accession was found that exhibits, in some vines and at whole plant level, completely white-skin bunches. Other vines exhibit branches giving rise to only white-skin berries and branches which present, in the same bunch, three different coloured berries (completely blue black or white berries and berries showing longitudinally white or blue black sectors). Genomic DNA was extracted from leaves and berries of all phenotypes and analyzed at six nuclear SSR (Simple Sequence Repeat) loci. The SSR analysis revealed that the fragments amplified (from all plants presenting bud sports) coincided in size with those from the Portuguese blue black-berried cultivar 'Alfrocheiro Preto'. The cultivar ancestry was further confirmed with ampelometric descriptors (OIV, 2007) and phenological observations. We infer that the white-berried plants are a bud sport arisen in 'Alfrocheiro Preto' through a somatic mutation.