Cloning and sequence analysis of glycolate oxidase homologues gene (LcGOX2) from litchi pericarp.

摘要

A full cDNA sequence encoding glycolate oxidase (GOX) was first isolated from litchi pericarp using DDRT-PCR, which was named LcGOX2. The full-length cDNA of LcGOX2 was 1104 bp, encoding 367 amino acids. Sequence analysis showed that LcGOX2 had a high homology in amino acid sequence to previously recorded plant GOX genes and shared the highest (99%) identity with Arabidopsis GOX2. Phylogenetic analysis based on more than 30 GOX amino acid sequences available using DNAstar software suggested that LcGOX2 sequence was the most close to Arabidopsis GOX2 gene. The calculated molecular weight, amino acid composition, hydrophilic and protein structure were also predicted using protein analysis software online (http://cubic.bioc.colubia.edu) and BioEdit software.