Investigation of the factors affecting cross-fertilization rate in rose.

摘要

Rose has the longest history of artificial improvement by man-kind in ornamental plants. Owing to their complex genetic constitution, rose is heavily self-incompatible and gives low cross-fertilization rates by crossing genetically distant pairs. Consequently, low cross-fertilization rates results in a negative effect on the breeding process. In this study, we investigated the factors that would influence the cross-fertilization rate of roses. Correlation analysis was performed between cross-fertilization rate and genetic distances of the parents. Analysis of variance was also performed to study the paternal and maternal effects for the cross-fertilization rate. Reliable data for cross-fertilization rate were obtained from Jeollanamdo Agricultural Research and Extension Services, Korea in 2006. 32 Cross combinations provided cross-fertilization rates. Their genetic distances were obtained by using RAPD marker analysis. In RAPD analysis, we used 16 primers and obtained clear 101 RAPD markers. Genetic distances were obtained by using Nei's coefficients. Relationship between cross-fertilization rate and genetic distance of parents was conducted by using correlation analysis. The correlation coefficient as low as 0.058, was not significant. Consequently, we determined that overall genetic distances do not influence the cross-fertilization rate by any means. The paternal and maternal effects for the cross-fertilization rate were determined. The results of analysis of variance showed that the cross-fertilization rate is only influenced by the maternal side. This shows that rose breeders must have desirable materials that can be mother plants. Moreover, we expect that more complicated factors will influence the cross-fertilization rate. Further research should be carried out to elucidate this matter.