In vitro multiplication of highbush blueberry ( Vaccinium corymbosum L.) cultivars.

摘要

The purpose of this study was to develop an efficient in vitro multiplication system for highbush blueberry cultivars 'Blueray', 'Bluecrop' and 'Berkeley'. The three genotypes were successfully established in vitro using 0.15% mercuric chloride as a sterilization solution. Anderson's rhododendron medium (AN), half-strength Murashige and Skoog medium (half-MS) and McCown woody plant medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg L-1 were tested. Anderson's rhododendron medium and McCown woody plant medium proved to be suitable for cultivation of selected highbush blueberry cultivars in vitro. The highest multiplication 4.8+or-0.2 was noted for 'Berkeley' on McCown woody plant medium with zeatin (2 mg L-1). The lowest multiplication was noted for all cultivars on half-strength Murashige and Skoog medium.