The study of increasing roots shoots and hairy roots production by different extracts of saffron (Crocus sativus L.) in mungbean (Vigna radiata) seedlings.

摘要

Mungbean (Vigna radiata) is a dicotyledonous plant from leguminosea family. Recent pharmacological studies have demonstrated that saffron (Crocus sativus L.) extract has antitumor and hypolipidemic effects but in this investigation, we studied effects of extracts of saffron on growth of mungbean seedlings. In the maceration method, the stigmas were macerated in warm distilled water for three days. The extract was subsequently filtered and the remains of stigmas were washed 10 times. The remains of stigmas were macerated in 100 ml of acetone for one day and it was filtered and concentrated under reduced pressure at 35 degrees C. The acetonic extract was solved in 100 ml of distilled water. The seeds of mungbean (100 seeds) were cultured in 15-20 ml of these two extracts with different concentrations. After two weeks, results of these experiments indicated that the treatment increased the rate of shoots, roots growth and hairy roots production but it reduced the diameter of shoots and roots. The extracts of saffron are growth stimulator in mungbean seedlings.