Marker-assisted selection of self-incompatibility allows an efficient double cross breeding in radish (Raphanus sativus L.).

摘要

Self-incompatibility (SI) plays an important role in seed production of commercial hybrid cultivars in Cruciferae vegetables for preventing self-pollination, but this trait also makes it difficult to proliferate both parental lines and hybrid cultivars of radish. A new breeding method was developed to increase the uniformity of F1 hybrid seeds and cut the cost of seed production using near-isogenic lines on double cross. To make near-isogenic lines, polymerase chain reaction (PCR) amplification was used for selecting plants heterozygous for the S-locus. Plants possessing different alleles on the S-locus were PCR amplified with two kinds of class specific primer pairs. One of the problems of applying PCR markers on conventional breeding was the time and cost needed for extracting DNA. Results demonstrated that the effective method of DNA extraction was using 'FB plate' and the PCR-based selection on S-locus was found to be a feasible tool for developing new breeding methods for enhancing purity and increasing production of hybrid seeds..