Description of the lake baikal microorganism communities growing on a steel plate and in the natural water surrounding this substratum

摘要

This article presents an analysis of the microbial community growing on a stainless steel plate. A sample from this plate has been tested for the rpoC gene. Dominant biofilm species (clones) were identified with the help of molecular cloning and nucleotide sequencing of a 16S rRNA gene fragment. The activities of planktonic microorganisms and biofilm microorganisms attached to the plate were compared. Seven of nineteen sequences (37%) were found to belong to the Sphingobacteria group. Analysis of 16S rRNA gene sequences cloned from natural biofilms sampled from Lake Baikal showed a great variety of microorganisms in the biofilm. The following taxonomic groups were revealed among them: sphingobacteria, alpha-proteobacteria, cyanobacteria, verrucomicrobia, bacilli, gamma-proteobacteria, flavobacteria, and beta-proteobacteria. This kind of analysis was done in Lake Baikal for the first time.