Ecological and geographic variability of Hedysarum theinum and features of its propagation in vitro

摘要

The relationship between the morphological variability of the productivity characteristics and the genetic variability of the electrophoretic spectra of seed polypeptides in the valuable medicinal species Hedysarum theinum Krasnob was established based on analyzing six cenopopulations. The protocols for the microclonal propagation of advanced samples were developed. The most efficient multiplication was achieved in an MS medium supplemented with 5 mu M BAP, 200 mg/L-1 glutathione, and 200 mg/L-1 casein hydrolyzate. The shoots were successfully rooted on the half-strength MS basal medium supplemented with 7 mu M NAA.