Silencing of Human Choline Kinase Expression: Quantification Using Transcriptor First Strand cDNA Synthesis Kit and the LightCycler System

摘要

RNA interference (RNAi) has emerged as a powerful method for silencing expression of a gene of interest. Direct transfection of the desired cell type with siRNA molecules is a fast approach to transient gene silencing and allows one to track effects such as phenotypic changes up to several days following transfection [1].