Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes

摘要

The following protocol was optimized from a protocol using ~(35)S-labeled RNA probes [1, 2]. In research studies, it enables detection of the expression of rare mRNAs in the cardiovascular system (e.g., of the proinflammatory cytokine granulocyte macrophage colony stimulating factor [GM-CSF] in normal human coronary arteries, and of interleukin 6 [IL6] and glycoprotein gp130 in failing human hearts [3]). The protocol can be combined with immunohistochemistry [4, 5], and can also be used for paraffin-and methacrylate-embedded sections.