Automated DNA Isolation from Genetically Modified Soybeans and Soybean Derived Food Material with the MagNA Pure LC System

摘要

Worldwide, the production of genetically modified organisms (GMO) is increasing rapidly. In Japan, a country that imports many concern. Therefore, the construction of a fast and reliable inspection system is demanded. For this purpose, we have developed a new high throughput method for DNA isolation of GMOs from food. The method contains two steps: →manual pretreatment using an extraction buffer →automated DNA isolation performed with the MagNA Pure LC DNA Isolation Kit The method was tested with the raw soybean material and a variety of food products containing soybeans. The tests included criteria like DNA integrity, yield, purity, reproducibility and scalability.