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首页> 外文期刊>Conservation genetics >Isolation and characterization of 11 microsatellite loci in Scotophilus kuhlii (Lesser Asiatic Yellow House Bat)
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Isolation and characterization of 11 microsatellite loci in Scotophilus kuhlii (Lesser Asiatic Yellow House Bat)

机译:苦瓜(Scotophilus kuhlii)(Lesser Asiatic Yellow House Bat)中11个微卫星基因座的分离和鉴定

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摘要

We used enriched genomic library method to isolate and characterize 11 di-nucleotide microsatellite loci in the Lesser Asiatic Yellow House Bat, Scotophilus kuhlii. The polymorphism of these loci was tested on a natural population of 36 individuals from Hainan Province, South China. All loci revealed the polymorphism ranging from 5 to 18 alleles. The observed heterozygosity values were from 0.694 to 1.000 and expected heterozygosity values were from 0.558 to 0.929. No significant linkage disequilibrium was detected between any pairs of loci. These polymorphic markers will be used to assess population structure in S. kuhlii.
机译:我们使用丰富的基因组文库方法来分离和鉴定小亚细亚黄房子蝙蝠Scotophilus kuhlii中的11个二核苷酸微卫星基因座。这些基因座的多态性在来自中国南方海南省的36个自然种群上进行了测试。所有基因座都揭示了5至18个等位基因的多态性。观察到的杂合度值为0.694至1.000,预期杂合度值为0.558至0.929。在任何对基因座之间均未检测到明显的连锁不平衡。这些多态性标记将用于评估库氏链球菌的种群结构。

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