CHO Cell Culture: Single-Use CelliGen~R BLU Packed-Bed Fibra-Cel~R Basket

摘要

Packed-bed basket technology developed by New Brunswick Scientific (acquired by Eppendorf Inc. in 2007) provides a shear-free environment for production of animal cells. Because little information is available on the utility of the New Brunswick CelliGen BLU single-use bioreactor system for production of secreted proteins (especially in perfusion mode), this study was conducted to measure growth and productivity of alkaline phosphatase (ALKP)-secreting rCHO cells. We used two packed-bed bioreactor types — a 5-L New Brunswick CelliGen BLU single-use vessel and a 2.5-L autoclavable glass vessel — both operated by New Brunswick CelliGen 310 console in perfusion mode. Perfusion provides a homeostatic environment for optimal cell growth similar to thatexperienced by cells in vivo, where waste products are constantly removed and fresh nutrients are replenished. Cells cultured in packed-bed bioreactors are not exposed to hydrodynamic forces, which allows for maximum cell growth and protein expression (l). Our objective was to determine whether any differences are observed between the productivity of the two bioreactors.