Culture of Normal Human Dermal Fibroblast Cells in a Functionally Closed, Automated, Cell-Expansion System

摘要

The large numbers of ex vivo expanded cells that are required in many clinical cell therapy protocols (>100 million per patient) make standard culture conditions problematic and expensive, resulting from the need for extensive personnel and facilitiesresources and the high potential of contamination. To meet such clinical demand, a robust, automated, and closed cell-expansion method is optimal. The Quantum Cell Expansion System (CES) is a functionally closed, automated, hollow-fiber bioreactor system designed to reproducibly grow both adherent and suspension cells in either GMP or research laboratory environments. The Quantum GES has successfully been used for the ex vivo expansion of clinical-scale quantities of adult bone marrow-derived mesenchymal stem cells (MSCs). It has now been demonstrated that a second adherent cell type of clinical interest, adult normal human dermal fibroblasts (NHDF), are ex vivo expandable with the Quantum CES.