Solo Hill Microcarriers in a 25-L PadReactor~(TM) Single-Use Bioreactor

摘要

The purpose of this study was to demonstrate compatibility and performance of SoloHill's Collagen-coated microcarriers in ATMI Lifesciences' Integrity~(TM) PadReactor~(TM) system. The solid polystyrene core of the collagen microcarriers prevents absorption of serum or cell products into the core of the microcarrier, and the 1.03 specific gravity and 125- to 212-pm diameter allows them to be maintained in suspension with gentle low stirring speed. These solid microcarriers provide an excellent substrate for cell growth. They can be sterilized by autoclaving at temperatures up to 131 °C or by gamma irradiation at doses of 25-40 kGy with no deleterious effects on function.