Expression of a Fab Fragment in Pfenex Expression Technology~(TM): Comparison with Escherichia coli and Pichia pastoris

摘要

The Pfenex Expression Technology~(TM) platform is a powerful protein expression technology based on the novel host Pseudomonas fluorescens. Legacy recombinant protein expression hosts such as E. coli and yeast tend to have high failure rates with regard to expression of soluble, active protein. In addition, the time required to identify the optimal expression strain in these systems is protracted, resulting in significant opportunity cost for drug developers. Leveraging the Pfenex Expression Technology~(TM) shortens development time via higher success rates and lowers overall cost of goods associated with rapid process cycle times, high cell densities, and low-cost media.