Optimizing Cryopreservation for Therapeutic Cells

摘要

Biopreservation suppresses degradation and enables postpreservation recovery of structure, viability, andfunction. Although there are several biopreservation techniques (indicated in "Biopreservation Methods" box), most laboratories use either standard cryopreservation protocols (the far majority) or vitrification (much more limited in broad systems application) when freezing cells for research and clinical applications. Isopropanol freezing containers such as the Mr. Frosty device from Nalgene Labware have made cryopreservation easier in many applications, and controlled-rate freezers allow users to program and manipulate freezing rates.