Simultaneous saccharification and co-fermentation of un-detoxified rice hull hydrolysate by Saccharomyces cerevisiae ICV D254 and Spathaspora arborariae NRRL Y-48658 for the production of ethanol and xylitol

摘要

Co-fermentation and simultaneous saccharification of rice hull hydrolysate (RHH) were investigated for the production of ethanol and xylitol by Saccharomyces cerevisiae, Spathaspora arborariae, or the combination of both. In bioreactor cultures under oxygen limitation, S. cerevisiae was capable of metabolizing glucose from RHH, which contained small amounts of acetic acid, furfural, and hydroxymethylfurfural, achieving ethanol yields of 0.45 and concentrations of 10.5gL~(-1). In the co-culture of S. cerevisiae and S. arborariae pentoses and hexoses from RHH, were converted to ethanol and xylitol, with yields of 0.48 and 0.39, and concentrations of 11gL~(-1) and 3gL~(-1), respectively. The simultaneous saccharification and co-fermentation using both yeasts produced ethanol and xylitol to final concentrations of 14.5gL~(-1) and 3gL~(-1), respectively. Results showed good prospects to use co-cultures of S. cerevisiae and S. arborariae for the bioconversion of RHH into ethanol and xylitol without further detoxification.