Large Scale CHO Transient Gene Expression Using Flow ElectroporationAntibody Titers 1 g/L Using MaxCyte Transfection and Optimized Feeding

摘要

Researchers have turned to Chinese hamster ovary (CHO)-based transient gene expression (TGE) as an alternative to CHO stable cell line production for early stage antibody development. Despite advances in transfection methods and culture optimization,most CHO-based TGE systems produce antibody titers (low mg/L) that are insufficient for full use in biotherapeutic development pipelines. MaxCyte electroporation is a universal, transient transfection platform with unmatched quality, flexibility, and scalability and the capacity to transfect up to 2 x 1011 cells in under 30 minutes. CHO transfection efficiencies and viabilities of >90% generate antibody titers over 400 mg/L within two weeks of transfection and can exceed 1 gram/L with optimization. MaxCyte electroporation can simultaneously create high-yield, stable CHO cell lines (>3 g/L productivity) in six to eight weeks using a simple selection protocol, thereby streamlining scale up to clinical-grade biomanufacturing.