Emerging Challenges to Protein A: Chromatin-Directed Clarification Enables New Purification Options

摘要

Protein A affinity chromatography has been a target for replacement since its commercial debut, mainly because of its high acquisition cost. The technique became established despite the cost because it was born into an industrial culture that favoredspeed to market over manufacturing economy (l). Vendors have since strengthened protein A's position with incremental but worthy improvements such as higher capacity, lower ligand leaching, and modest tolerance of NaOH. Collateral improvements in polishing technologies, such as the high throughput and favorable economics of single-use membrane adsorbers, have helped reduce overall purification costs.