Multiple -Step Kineic Mechanisms of the ssDNA Recognition Process by Human Polymerase #beta# in Its Different ssDNA Binding Modes

摘要

The kinetics of human polymerase #beta# (pol #beta#) binding to the single -stranded DNA, in the (pol#beta#)_(16) and (pol #beta#)_5 binding modes, that differ in the number of occluded nucleotide tesidues i the protein-DNA complexes, have been examined, using the fluorescence stopped -flow technique