Resolution of Michaelis Complex , Acylation , and Conformational Change Steps in the Reactions of the Serpin , Plasminogen Activatro Inhibitor-1 with Tissue Plasminogen Activator adn Trypsin

摘要

Michaelis complex , acylation, and conormational change steps were resolved in the reactions of the serpin plasminogen activator inhibitor-1(PAI-1) , with tissue plasminogen activator (tPA) and trypsin by comparing the reactions of active and Ser 195 -inactivated enzymes with site-specific flulorecent labeled PAI-1 derivatives that report these events