Magnetic Isotope of Magnesium Accelerates ATP Hydrolysis Catalyzed by Myosin

摘要

In this paper, we present the results of experimental studies on the influence of different magnesium isotopes, magnetic ~(25)Mg and nonmagnetic ~(24)Mg or ~(26)Mg, on ATP-hydrolytic activity of the isolated myosin subfragment-1. The reaction rate in the presence of magnetic ~(25)Mg isotope turned out to be 2.0-2.5 times higher than that using non-magnetic ~(24)Mg or ~(26)Mg isotopes. In absence of the enzyme, as at spontaneous ATP hydrolysis in aqueous solution, no magnetic isotope effect was observed. Thus, a significant catalytic effect of the magnetic ~(25)Mg isotope (nuclear spin catalysis) was discovered in the enzymatic hydrolysis of ATP.