The Effect of NT-1505 on the Structure of Endoplasmic Reticulum Membranes in vivo

摘要

The effect of NT-1505 on the membranes of the endoplasmic reticulum was studied. It is shown that the dynamics of changes in the microviscosity of lipid and near-protein regions of endoplasmic reticulum membranes with the administration of the preparation is antibate in character, which indicates the absence of pathological changes in the membrane structure. The microviscosity of membranes was measured by electron paramagnetic resonance of 2,2,6,6-tetramethyl-4-capryloyl-oxylpiperidine-1-oxyl (lipid probe) and 5,6-benzo-2,2,6,6-tetramethyl-1,2,3,4-tetrahydro-γ-carboline-3-oxyl (near-protein probe) spin probes. In order to obtain more information about the changes in the structure of membranes under the action of the NT-1505 neuroprotector, the temperature dependence of the correlation time of rotational diffusion was measured in the temperature range of 283–317 K (10–44°C). In the control group, two structural transitions in the temperature ranges of 16–20 and 32–38°C were characterized for both areas of membranes, which remained following the introduction of NT-1505. Thus, NT-1505 does not significantly affect the structure of the endoplasmic reticulum membrane.