Decomposition of the Hemoglobin UV Absorption Spectrum into the Absorption Spectra of a Prosthetic Group and Apoprotein Using an Additive Model

摘要

The decomposition pathways of the hemoglobin UV-absorption spectrum into the absorption spectra of the protein and non-protein components were proposed and substantiated by means of an additive model. We have established that the heme component has an absorption band with a maximum at λ_(max) = 269.2 nm (ε = 97163) and the apoprotein component has an absorption band with a maximum at λ_(max) = 278.4 nm (ε = 48669) for the wavelength range from 240.0 to 320.0 nm. The integral relative proportion of absorption for the heme fraction (78.8%) and apoprotein (21.2%) in the investigating wavelength range was determined.