The Process of Heme Synthesis in Bone Marrow Mesenchymal Stem Cells Cultured with Fibroblast Growth Factor bFGF and under Hypoxic Conditions

摘要

It was shown that fibroblast growth factor bFGF influences the process of heme synthesis and the proliferative activity and viability of bone marrow mesenchymal stem cells in culture under hypoxic conditions. The addition of fibroblast growth factor bFGF (7 ng/mL) to the medium under the above conditions led to the accumulation of aminolevulinic acid (an early porphyrin and heme precursor), an increase in the expression of CD71 (transferrin receptor), and a decrease in the content of porphyrin pigments and heme (a late precursor and end product of heme synthesis, respectively). It was found that cultivation of the cells under hypoxic conditions and bFGF is optimal for maintaining high viability and proliferation capacity of the mesenchymal stem cells.