Design of Experiments for Fed-Batch Process Development in Shaken Cultures:Determining Reproducibility and Scalability

摘要

When designing a recombinant protein production process, a high number of parallelcultivations must be carried out. That task is typically performed using batch cultures in shake flasks or microwell plates, in which fermentation conditions are not monitored. To overcome that limitation, we combined the SensorDish Reader and Shake Flask Reader systems (from PreSens) with an enzymatic glucose delivery system (EnBase technology from BioSilta Oy) for Escherichia coli cultivations. Our objective was to determine whether SensorDish reader cultures would yield reproducible process data thatwere directly comparable to those at shake flask scale (Photo 1).