Morphologies of PC12 Cells Cultured on Some Polymeric Films—Relationship betweenCell Growth and Surface Physical Properties—

摘要

The growth and spreading of P012 pheochromocytoma cells, on various polymeric films prepared by Langmuir—Blodgett (LB) and casting methods were investigated. P012 cells, which were cultivated on collagen and synthetic polymeric films prepared by the LB method, adhered and spread much more than those on the films prepared by the casting method. The relationship between the cell growth or spreading and physical properties (i.e, contact angle and surface roughness) of the polymeric films where the cells were cultivated was investigated. Exponential relationship was observed in the plots of the cell density versus root mean square of roughness of the films, which was estimated by atomic force microscopy. The cell growth and ~preading ratio of P012 cells were found to be higher on the LB films which adsorbed the serum proteins containing higher content of a—helix than those on the casting films. The contact angle of LB films was found to be different from that of cast films prepared from the same polymer used in the LB films. The maximum cell density and spreading ratio were observed on the films having a contact angle of around 55 degrees. It is suggested that the adsorbed serum proteins having high a-helix contents on the films control and stabilize the proliferation and spreading of P012 cells cultivated on the films.