Investigation of Changes in Fluidity of Brain Membranes in Mice Bearing Experimental Alzheimer’s Disease Using Spin Labeling Electron Paramagnetic Resonance

摘要

which were obtained from forebrain tissue of mice bearing experimental Alzheimer’s disease induced with olfactory bulbectomy were studied. Membrane microviscosity was measured by electron paramagnetic reso- nance with 2,2,6,6-tetramethyl-4-capryloyl-oxypiperidine-1-oxyl and 5,6-benzo-2,2,6,6-tetramethyl- 1,2,3,4-tetrahydro-γ-carboline-3-oxyl as spin labeling. Phasic alterations in relative fluidity were found dur- ing the development of experimental Alzheimer’s disease pathology. These changes correlate with the stages of “clinical” features. Main abnormalities were related to structural changes in near-protein region of the lipid bilayer.