Hematological, serum biochemical parameters, and physiological responses to acute stress of Beluga sturgeon (Huso huso, Linnaeus 1785) juveniles fed dietary nucleotide

摘要

The present study aimed to investigate the effects of dietary nucleotide on serum biochemical and hematological parameters as well as stress response in Beluga sturgeon Huso huso juveniles. Fish (12. 6 ± 0. 5 g) were fed diets containing 0%, 0. 15%, 0. 25%, 0. 35%, and 0. 50% nucleotide over a 62-day period. The experiment was carried out in 600-L tanks with 30 fish per tank. Fish were fed five times a day until apparent satiation. At the end of the experiment, blood samples were collected from treatments for investigation of biochemical and hematological parameters. Then, fish were subjected to an acute stress. Blood samples were collected at 0 and 6 h post stress, and plasma cortisol and glucose levels were determined. The results showed that total protein, cholesterol, triglycerides, albumin, and globulin were not affected by dietary nucleotide (P > 0. 05). Hematocrit and hemoglobin values were significantly higher in the 0. 35% treatment compared to those of the control (P < 0. 05). Beluga juveniles fed 0. 5% dietary nucleotide had a significantly lower hemoglobin value (P < 0. 05). Lymphocyte proportion was significantly higher in the 0. 25 and 0. 35 nucleotide groups than in the control group (P < 0. 05). Cortisol and glucose levels in all groups were significantly affected by time; nevertheless, in fish fed nucleotides, stress-induced cortisol elevation was lower compared to that of the control group (P < 0. 05). The results indicated that dietary nucleotide supplementation was capable of affecting hematological parameters and physiological stress responses of Beluga juveniles.