Effect of a 50-Hz electromagnetic field on the gene expression of glutathione S-transferase Tl (Gsttl) in the testis and liver of male rats

摘要

Glutathione S-transferases (GSTs) are a super-family of dimeric proteins catalyzing the conjugation of glutathione with a wide variety of compounds and provide a strong antioxidant function by neutralizing electrophiles and free radicals. The subunits of the GSTs family are placed into several classes including theta (GST0). The aim of this study was to demonstrate the expression of Gsttl (a member of GST0) gene in the testis and liver of rat in response to extremely low-frequency electromagnetic fields (ELF-EMF). After exposing male Wistar rats to the stressor (ELF-EMF 50 Hz, 500 uT) for 30 days, we quantified the gene expression of the Gsttl by reverse transcription polymerase chain reaction in exposed and unexposed rats. Results indicated that the expression of Gsttl in the liver and testis did not undergo any change at transcription level by environmental exposure to ELF-EMF at this duration and intensity.