Effect of estradiol on stimulated theophylline and prolactin Ca2+ exit from intracellular stores of pig oocytes.

摘要

The effect of estradiol on theophylline- and prolactin-stimulated Ca2+ exit from intracellular stores of pig oocytes was investigated using fluorescent dye chlortetracycline. In the presence of estradiol, neither theophylline nor prolactin stimulated Ca2+ exit from intracellular stores of oocytes. However, in the absence of estradiol, both theophylline and prolactin stimulated Ca2+ exit from intracellular stores. Inhibition of protein kinase C also inhibited Ca2+ exit from intracellular stores. It is concluded that estradiol inhibits theophylline- and prolactin-stimulated Ca2+ exit from intracellular stores of pig oocytes.