Response by T. Palosuo, N. Kalkkinen, H. Alenius, R. G. Hamilton and C. Blanco

摘要

We would like to thank Dr Yeang for his interest and careful review of our article. He asks whether it is possible that our proteins were 'over-purified' and suggests that due to extensive purification we could have removed from the final preparations some important isoforms with IgE-binding properties. At a general level concerning purification of proteins, our methods represent well-established protein chemistry techniques that are aimed at purifying proteins in their native conformation. A major focus of our work has been to eliminate other possible interfering allergens.