Micro-Western Array (MWA), a novel proteomics platform, is composed of GeSim Nanoplotter protein array, GE Multiphor, and Licor Odyssey infrared scanner. MWA is a high-throughput Western blotting assay which is able to detect the abundance and phosphorylated status of 384 proteins in 6 samples simultaneously. Including electrophoresis procedure allows MWA to avoid antibody cross-reactivity, which is a common problem in protein array. MWA is most useful for signaling transduction studying, molecular mechanism investigation, biomarker screening, and antibody validation. MWA will be a very useful tool for systems biology and biomedical research.
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