An examination method for chemiluminescence of whole-blood leucocytes

摘要

The chemiluminescence examination methods have been limited in use due to a lack of standard techniques for the preparation and storage of biological samples. The methods of sampling the blood and the conditions of its storage and transportation as well as other factors essentially affect the functional state of cells through activating them. The suggested technique keeps the values of blood chemiluminescence stable for 2 hours in measurements of spontaneous chemiluminescence and for 6 hours in measurements of stimulated chemiluminescence. For the purpose, whole blood is mixed, in test-tubes, with a 0.27% solution of disodium EDTA. It is stored in refrigerator at 0 to 5 degrees C. Blood is diluted by Henk solution and luminol for measurements.