Development of disposable biosensor system based on chemically modified screen-printed bilayer electrodes and application to estimation of fish freshness.

摘要

A disposable biosensor system based on bilayer electrodes using a screen-printing technique has been developed and applied to the simple and rapid determination of fish freshness (K value). HxR and Hx were measured by the upper layer electrode (EL1). IMP,HxR and Hx were measured by the lower layer electrode (EL2). The K value was determined by calculating the ratio of the outputs of the two electrodes (EL1/EL2). The K value was linearly correlated with the electrode output ratio from 10 to 90% with a very high correlation coefficient (R{sup}2) of 0.9933. One assay could be completed within 5min. The proposed biosensor system was stable for 30 days under drying and refrigeration condition.