PRE-DECIDUAL MULTIPOTENT STROMAL CELLS (PreDMSC) CONSTITUTIVELY EXPRESS PROGESTERONE INDUCED BLOCKING FACTOR (PIBF)

摘要

Progesterone induced blocking factor (PIBF) has been described as an active factor which is intimately involved in regulation of the immune response in pregnancy. This 34-kDa protein which is released by progesterone-treated peripheral and placental lymphocytes decreases the NK cells cytotoxicity and prevents fetal rejection. Pre-decidual multipotent stromal cells (pre-DMSCs) are present in human decidua and are also involved in the local regulatory mechanisms of the successful pregnancy. The aim of this study was to detect the expression of PIBF in pre-DMSCs cultured in the presence of progesterone acetate (PgAc). Our data showed that Pg used in physiological for pregnancy doses decreased the percentage of cells positive for intracellular PIBF in pre-DMSCs. While high homogeneous positive staining was observed in control cells, PgAc-treated pre-DMSC showed a low amount of granular perinuclear staining. We suggest that low amounts of PIBF in PgAc-treated pre-DMSCs might be associated to the release of this protein out of the cells under hormone influence.